You can download the EICAR test file from the following Web site:
Alternatively, you can create your own EICAR test file by typing or copying the following characters into a text file, and then save the file with a com extension (for example, virus.com):
X5O!P%@AP[4\PZX54(P^)7CC)7}$EICAR-STANDARD-ANTIVIRUS-TEST-FILE!$H+H*
You may need to disable HTTP scanning, if any, before downloading the file. Include the test file as an email attachment to test SMTP scanning, and to check FTP and HTTP file transfers, for example, if you have Trend Micro InterScan VirusWall™ installed on the network.
For either option, the mere downloading or creation of the file should be enough to trigger real-time scanning.